Ubiquitin-dependent proteolysis in yeast cells expressing neurotoxic proteins

Critically impaired protein degradation is discussed to contribute to neurodegenerative disorders, including Parkinson’s, Huntington’s, Alzheimer’s, and motor neuron diseases. Misfolded, aggregated, or surplus proteins are efficiently degraded via distinct protein degradation pathways, including the ubiquitin-proteasome system, autophagy, and vesicular trafficking. These pathways are regulated by covalent modification of target proteins with the small protein ubiquitin and are evolutionary highly conserved from humans to yeast. The yeast Saccharomyces cerevisiae is an established model for deciphering mechanisms of protein degradation, and for the elucidation of pathways underlying programmed cell death. The expression of human neurotoxic proteins triggers cell death in yeast, with neurotoxic protein-specific differences. Therefore, yeast cell death models are suitable for analyzing the role of protein degradation pathways in modulating cell death upon expression of disease-causing proteins. This review summarizes which protein degradation pathways are affected in these yeast models, and how they are involved in the execution of cell death. I will discuss to which extent this mimics the situation in other neurotoxic models, and how this may contribute to a better understanding of human disorders

Threading Granules in Freiburg : 2nd International Symposium on "One Mitochondrion, Many Diseases – Biological and Molecular Perspectives", a FRIAS Junior Researcher Conference, Freiburg im Breisgau, Germany, March 9th/10th, 2016

Altered mitochondrial activities play an important role in many different human disorders, including cancer and neurodegeneration. At the Freiburg Institute of Advanced Studies (FRIAS) Junior Researcher Conference “One Mitochondrion, Many Diseases – Biological and Molecular Perspectives” (University of Freiburg, Freiburg, Germany), junior and experienced researches discussed common and distinct mechanisms of mitochondrial contributions to various human disorders

Hadro-Chemistry and Evolution of (Anti-) Baryon Densities at RHIC

The consequences of hadro-chemical freezeout for the subsequent hadron gas evolution in central heavy-ion collisions at RHIC and LHC energies are discussed with special emphasis on effects due to antibaryons. Contrary to naive expectations, their individual conservation, as implied by experimental data, has significant impact on the chemical off-equilibrium composition of hadronic matter at collider energies. This may reflect on a variety of observables including source sizes and dilepton spectra.Comment: 4 pages ReVTeX incl. 3 ps-figs, submitted to PR

Temperature-induced pair correlations in clusters and nuclei

The pair correlations in mesoscopic systems such as $nm$-size superconducting clusters and nuclei are studied at finite temperature for the canonical ensemble of fermions in model spaces with a fixed particle number: i) a degenerate spherical shell (strong coupling limit), ii) an equidistantly spaced deformed shell (weak coupling limit). It is shown that after the destruction of the pair correlations at T=0 by a strong magnetic field or rapid rotation, heating can bring them back. This phenomenon is a consequence of the fixed number of fermions in the canonical ensemble

Dileptons in High-Energy Heavy-Ion Collisions

The current status of our understanding of dilepton production in ultrarelativistic heavy-ion collisions is discussed with special emphasis on signals from the (approach towards) chirally restored and deconfined phases. In particular, recent results of the CERN-SPS low-energy runs are compared to model predictions and interpreted. Prospects for RHIC experiments are given.Comment: Invited talk at ICPAQGP, Jaipur, India, Nov. 26-30, 2001; 1 Latex and 9 eps-/ps-files Reoprt No.: SUNY-NTG-02-0

Thermal Relaxation of Charm in Hadronic Matter

The thermal relaxation rate of open-charm ($D$) mesons in hot and dense hadronic matter is calculated using empirical elastic scattering amplitudes. $D$-meson interactions with thermal pions are approximated by $D^*$ resonances, while scattering off other hadrons ($K$, $\eta$, $\rho$, $\omega$, $K^*$, $N$, $\Delta$) is evaluated using vacuum scattering amplitudes as available in the literature based on effective Lagrangians and constrained by realistic spectroscopy. The thermal relaxation time of $D$-mesons in a hot $\pi$ gas is found to be around 25-50\,fm/$c$ for temperatures $T$=150-180\,MeV, which reduces to 10-25\,fm/$c$ in a hadron-resonance gas. The latter values, argued to be conservative estimates, imply significant modifications of $D$-meson spectra in heavy-ion collisions. Close to the critical temperature ($T_c$), the spatial diffusion coefficient ($D_s$) is surprisingly similar to recent calculations for charm quarks in the Quark-Gluon Plasma using non-perturbative $T$-matrix interactions. This suggests a possibly continuous minimum structure of $D_s$ around $T_c$.Comment: Figures modified; discussions and references adde

CHANG-ES XI: Circular Polarization in the Cores of Nearby Galaxies

We detect 5 galaxies in the CHANG-ES (Continuum Halos in Nearby Galaxies -- an EVLA Survey) sample that show circular polarization (CP) at L-band in our high resolution data sets. Two of the galaxies (NGC~4388 and NGC~4845) show strong Stokes $V/I\,\equiv\,m_C\,\sim\,2$\%, two (NGC~660 and NGC~3628) have values of $m_C\sim \,0.3$\%, and NGC~3079 is a marginal detection at $m_C\sim \,0.2$\%. The two strongest $m_C$ galaxies also have the most luminous X-ray cores and the strongest internal absorption in X-rays. We have expanded on our previous Faraday conversion interpretation and analysis and provide analytical expressions for the expected $V$ signal for a general case in which the cosmic ray electron energy spectral index can take on any value. We provide examples as to how such expressions could be used to estimate magnetic field strengths and the lower energy cutoff for CR electrons. Four out of our detections are {\it resolved}, showing unique structures, including a {\it jet} in NGC~4388 and a CP `conversion disk' in NGC~4845. The conversion disk is inclined to the galactic disk but is perpendicular to a possible outflow direction. Such CP structures have never before been seen in any galaxy to our knowledge. None of the galaxy cores show linear polarization at L-band. Thus CP may provide a unique probe of physical conditions deep into radio AGNs.Comment: 30 pages, 4 figures, accepted to MNRA

Transverse flow and hadro-chemistry in Au+Au collisions at \sqrt{s_{NN}}=200 GeV

We present a hydrodynamic assessment of preliminary particle spectra observed in Au+Au collisions at \sqrt{s_{NN}}=200 GeV. The hadronic part of the underlying equation of state is based on explicit conservation of (measured) particle ratios throughout the resonance gas stage after chemical freezeout by employing chemical potentials for stable mesons, nucleons and anti-nucleons. We find that under these conditions the data (in particular the proton spectra) favor a low freeze-out temperature of around 100 MeV. Furthermore we show that through inclusion of a moderate pre-hydrodynamic transverse flow field the shape of the spectra improves with respect to the data. The effect of the initial transverse boost on elliptic flow and the freeze-out geometry of the system is also elucidated.Comment: as published: more data included in Fig. 1, discussions throughout the text improved, 6 pages, 4 figure

Signatures of Thermal Dilepton Radiation at RHIC

The properties of thermal dilepton production from heavy-ion collisions in the RHIC energy regime are evaluated for invariant masses ranging from 0.5 to 3 GeV. Using an expanding thermal fireball to model the evolution through both quark-gluon and hadronic phases various features of the spectra are addressed. In the low-mass region, due to an expected large background, the focus is on possible medium modifications of the narrow resonance structures from $\omega$ and $\phi$ mesons, whereas in the intermediate-mass region the old idea of identifying QGP radiation is reiterated including effects of chemical under-saturation in the early stages of central Au+Au collisions.Comment: 17 pages ReVTeX including 16 figure